Group training

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Substrate & wood fungi production course

September 20 – 24, 2021

Fed up with the internet overload of information on mushroom growing? Don’t know who to believe anymore?

Then come and join one of our courses on the production of wood fungi such as Oyster mushroom, King Oyster (Eryngii), Shiitake, Hericium, Nameko and many other species. You will get a state-of-the-art overview of the current specialty mushroom industry.

We can not teach you how to grow White Button Mushrooms! For Agaricus bisporus trainings, check out Mushroom Office in the Netherlands. For Agaricus bisporus spawn production training, check out our spawn production training.

Over the years, many of you have come to our excellent training centre for mycelium and lignicolous substrate producers. Since November 2011, we are organising group trainings on the production of sterilized substrates and lignicolous mushrooms, which cover the whole production cycle from sawdust to specialty mushroom.

The program has been developed in close collaboration with Dipl. Ing. Jürgen Kynast, who has a broad-spectrum scientific background in combination with extensive experience as a grower and a substrate producer of specialty mushrooms. We welcome existing and would-be growers to this in-depth course.


Next edition: September 20 – 24, 2021
Fee: 1180 EUR per participant*
Location: Mycelia’s premises

Training language: English
Group size: maximum 21

*VAT, board and lodging not included.


08:30 – 08:40 Welcome and practical briefing
08:40 – 09:30 Parallel session: Introduction
     25 min      Group 1: Quiz slides Jan Van Nuffel
     25 min      Group 2: Mycelia floor plan and visit to the premises Kasper Moreaux
09:30 – 10:00 Coffee break
10:00 – 11:30 First introduction to substrate production: components, mixing, humidification, filling, bag closing, sterilization, cooling, spawning, incubation, growing systems Jürgen Kynast
11:30 – 12:30 Parallel session: Practical tests
     30 min      Group 1: Agar preparation (production of agar media, test with petridishes: testing hands and air) Pascale Vergeyle & Hélène Ntimba
     30 min      Group 2: Design your own substrate Kasper Moreaux
12:30 – 13:30 Lunch break
13:30 – 14:30 Biology part I: General biology, role of enzymes Magda Verfaillie
14:30 – 15:00 Coffee break
15:00 – 16:00 Parallel session: Production lab research
     20 min       Group 1: Applied research Mihály Vidacs
     20 min       Group 2: Visit to technical floor with overpressure units Magda Verfaillie
     20 min       Group 3: Pouring agar under LAF Hélène Ntimba
16:00 – 17:00 Biology part II: Bacteria and fungi (bacteria: cell structure and multiplication, perfect and imperfect Fungi: classification and life cycle) Magda Verfaillie
08:30 – 09:00 Quiz Magda Verfaillie
09:00 – 12:30 Parallel session: Ingredients and consumables
     60 min      Group 1: Practical substrate preparation in Mycelia’s workshop (selection of ingredients, filling of mixer, addition of water, examples of filling and mixing machines, pH and dry matter measurement) Jean Meirlaen & Pascale Vergeyle
10:00 – 10:30 Coffee break
     60 min      Group 2: Improving the structure of substrates (determination of water- and air-filled pores; characteristics and role of different additives: wood chips, wood shavings, etc; examples of substrate formulations on paper) Jürgen Kynast
     60 min      Group 3: Demonstration of incubating substrates (good and bad examples, discussion) Kasper Moreaux
12:30 – 13:30 Lunch break
13:30 – 15:00 Dealing with micro-organisms in substrate production:  presence of propagules in the air, cleanroom classes, air quality control, use of LAF, use of overpressure, rules of conduct Magda Verfaillie
15:00 – 15:30 Coffee break
15:30 – 16:30 Biological approach of different production stages: incubation, ripening, fructification Jürgen Kynast
16:30 – 17:00 Incubation and ripening phases: climate conditions, temperature vs. time Jürgen Kynast
08:30 – 10:00 Pests and diseases: prevention and solutions Jürgen Kynast
10:00 – 10:40 Spawn: spawn production and spawn quality, spawn testing, transport and conservation Magda Verfaillie
10:40 – 11:00 Coffee break
11:00 – 12:30 Autoclaving process: theory on steam production and heat transfer, correct autoclaving procedure, F- value, what happens in the substrate bags? Jürgen Kynast
12:30 – 13:30 Lunch break
13:30 – 15:30 Parallel session: Practical inoculation, machinery
     40 min      Group 1: Practical substrate inoculation in Mycelia’s technical lab (essentials of small-scale production inoculation, particle counting and air speed measurement) Jürgen Kynast & Magda Verfaillie
     40 min      Group 2: Practical substrate inoculation in Mycelia’s cleanroom (essentials of large-scale production inoculation) Kasper Moreaux & Hélène Ntimba
     40 min      Group 3: Autoclaves and steam generator (practical use of steam, valve and sterilisation cycle schematics) Pascale Vergeyle
15:30 – 16:00 Coffee break
16:15 – 17:00 Theory of pasteurization process Jürgen Kynast
08:30 – 09:20 Production technics: cooling and heating, racks and shelves Jürgen Kynast
09:30 – 10:30 Breathing bags: techniques and gas exchange Jan Van Nuffel
10:30 – 10:50 Coffee break
10:50 – 11:50 Parallel session
     30 min      Group 1: Construction and maintenance of laminar air flows (filter types, practicalities, dangers, practical review of LAF’s, build and efficiency) Kasper Moreaux
     30 min      Group 2: Cleanroom consumables and dressing procedure Magda Verfaillie
11:50 – 13:00 Fructification techniques: aeration systems, humidification systems, hygiene in fructification room, harvesting, mushroom quality, post-harvest care, spent substrate) Jürgen Kynast
13:00 – 14:30 Lunch break
14:30 – 15:15 Fructification phase: determination of best climate conditions Jürgen Kynast
15:15 – 15:45 Coffee break
     30 min      Group 3: Make the layout of a substrate annex mushroom production farm Jürgen Kynast
16:00 – 16:45 Building technics: construction materials, water supply, waste water, electricity, energy efficiency) Jürgen Kynast
16:45 – 17:00 Evaluation of Petri dishes made on day 1
6:30 Meeting outside the B&B De Steenhove
06:45 – 09:00 Drive to Verbruggen Paddenstoelen
09:00 – 12:00 Visit to Verbruggen Paddenstoelen: Pleurotus substrate producer and grower
12:00 – 12:40 Drive to Paddestoelenrijk
12:40 – 14:00 Lunch break
(lignicolous mushroom tasting, delivery of USB stick and certificate and closing of the course)
14:00 – 16:00 Visit to the Paddestoelenrijk: grower of various types of lignicolous mushrooms
16:00 – 18:30 Drive back to Belgium

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