Group training

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SPAWN production course

September 27 – 30, 2021

Trouble finding reliable information on spawn production? Stop looking on the internet and get the real deal now.

Since 1999, people from more than 70 countries have put their trust in our education program on spawn production. Because we have been spawn producers for more than 35 years. We share our knowledge with you.

Our 4-day program has been developed over more than 20 years of experience and is based on our extensive training experience from individual courses, group courses and substrate group trainings.

If you’re still in doubt, ask someone who has been here before. We give you the real deal, guaranteed. Warning: this is an in-depth course. Having some kind of scientific background is essential.


Next edition: September 27 – 30, 2021
Fee: 1480 EUR per participant*
Location: Mycelia’s premises

Training language: English. We can however accommodate for French, German and Spanish speaking participants to a certain degree.
Group size: maximum 21

*VAT, board and lodging not included.


08:30 – 09:00 Welcome and practical briefing
09:00 – 10:00 Mycelia introduction video, floor plan and visit to the premises Kasper Moreaux
10:00 – 12:00 Parallel session: Practical preparation
     50 min       Group 1: Practical spawn preparation (cooking, mixing and filling; water management; pH and dry matter measurement) Kasper Moreaux & Jean Meirlaen
     50 min       Group 2: Agar preparation (production of agar media, test with petridishes: testing hands and air) Pascale Vergeyle & Hélène Ntimba
10:50 – 11:10 Coffee break
12:00 – 12:40 First stages of spawn production (workshop, spawn production machinery, components, mixing, humidification, bag types, bag filling, sterilization) Kasper Moreaux
12:40 – 13:40 Lunch break
13:40 – 14:40 Biology part I: General biology, role of enzymes Magda Verfaillie
14:30 – 15:00 Coffee break
15:00 – 16:00 Parallel session: Sterile department
     30 min       Group 1: Practical agar pouring under LAF Pascale Vergeyle
     30 min       Group 2: Visit to the sterile department Magda Verfaillie
16:00 – 17:00 Biology part II: Bacteria and fungi (bacteria: Cell structure and multiplication, classification and life cycle of common fungi Magda Verfaillie


08:30 – 10:30 Disinfection and sterilization (methods and efficiency) Kasper Moreaux
10:30 – 10:50 Coffee break
10:50 – 11:40 Parallel session: Cleanroom essentials
     25 min       Group 1: Microscopy Magda Verfaillie
     25 min       Group 2: Consumables and use of USB microscope Kasper Moreaux
11:40 – 12:40 Parallel session: Essential machines
     30 min       Group 1: Autoclaves and steam generator, practical use of steam, cooling units Pascale Vergeyle
     30 min       Group 2: Visit to the technical floor Kasper Moreaux
12:40 – 13:40 Lunch break
13:40 – 14:40 Dealing with micro-organisms in the production of spawn (presence of propagules in the air, cleanroom classes, air control, use of LAF, use of overpressure, rules of conduct) Magda Verfaillie
14:40 – 15:00 Coffee break
15:00 – 16:20 Mother cultures and mother spawn (GMP, quality control and degeneration of strains) Kasper Moreaux
16:20 – 17:00 Case study: Examples from real life Pascale Vergeyle
08:30 – 13:00 Parallel session: Inoculation practice
     120 min       Group 1: Mother spawn department (pratical subculture method inoculation, strain preservation techniques, demonstration of incubating mother cultures) Magda Verfaillie & Hélène Ntimba
10:30 – 11:00 Coffee break
     120 min       Group 2: Spawn inoculation in Mycelia’s main inoculation room (essentials of large-scale production inoculation, particle counting and air speed measurement, incubation, shaking and reshaking, cleaning and hygiene) Pascale Vergeyle
13:00 – 14:00 Lunch break
14:00 – 14:30 Spawn conservation (spawn production and spawn quality, spawn testing, transport and conservation) Magda Verfaillie
14:30 – 15:30 Demonstration of incubating spawn (good and bad examples, discussion) Kasper Moreaux
15:30 – 16:00 Coffee break
16:00 – 17:00 Spawn bags (types of vessels, bags and boxes, essentials of filters, spawn bags vs. production system) Jan Van Nuffel
08:30 – 10:00 Feasibility study (financial study and investment) Roel Van de Heyning
10:00 – 11:20 Practical: Organization of a spawn lab Magda Verfaillie
11:20 – 11:40 Coffee break
11:40 – 12:40 Practical: Cleanroom class Jan Van Nuffel & Kasper Moreaux
12:40 – 13:40 Lunch break
13:40 – 16:00 Parallel session: Cleanrooms practical Pascale Vergeyle
     60 min      Group 1: Construction and maintenance of laminar air flows and overpressure units
     60 min      Group 2: Practical cleaning (materials, disinfection, water management and practical hygiene)
14:40 – 15:00 Coffee break
16:00 – 17:00 Evaluation of petridishes and multiple choice of day 1, delivery of USB stick and certificates

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