Group training
SPAWN production course
Trouble finding reliable information on spawn production? Stop looking on the internet and get the real deal now.
Since 1999, people from more than 70 countries have put their trust in our education program on spawn production. Because we have been spawn producers for more than 35 years. We share our knowledge with you.
Our 4-day program has been developed over more than 20 years of experience and is based on our extensive training experience from individual courses, group courses and substrate group trainings.
If you’re still in doubt, ask someone who has been here before. We give you the real deal, guaranteed. Warning: this is an in-depth course. Having some kind of scientific background is essential.
DETAILS
Next edition: September 27 – 30, 2021
Fee: 1480 EUR per participant*
Location: Mycelia’s premises
Training language: English. We can however accommodate for French, German and Spanish speaking participants to a certain degree.
Group size: maximum 21
*VAT, board and lodging not included.
Program
| SUBJECT | TRAINER | |
| 08:30 – 09:00 | Welcome and practical briefing | |
| 09:00 – 10:00 | Mycelia introduction video, floor plan and visit to the premises | Kasper Moreaux |
| 10:00 – 12:00 | Parallel session: Practical preparation | |
| 50 min | Group 1: Practical spawn preparation (cooking, mixing and filling; water management; pH and dry matter measurement) | Kasper Moreaux & Jean Meirlaen |
| 50 min | Group 2: Agar preparation (production of agar media, test with petridishes: testing hands and air) | Pascale Vergeyle & Hélène Ntimba |
| 10:50 – 11:10 | Coffee break | |
| 12:00 – 12:40 | First stages of spawn production (workshop, spawn production machinery, components, mixing, humidification, bag types, bag filling, sterilization) | Kasper Moreaux |
| 12:40 – 13:40 | Lunch break | |
| 13:40 – 14:40 | Biology part I: General biology, role of enzymes | Magda Verfaillie |
| 14:30 – 15:00 | Coffee break | |
| 15:00 – 16:00 | Parallel session: Sterile department | |
| 30 min | Group 1: Practical agar pouring under LAF | Pascale Vergeyle |
| 30 min | Group 2: Visit to the sterile department | Magda Verfaillie |
| 16:00 – 17:00 | Biology part II: Bacteria and fungi (bacteria: Cell structure and multiplication, classification and life cycle of common fungi | Magda Verfaillie |
| SUBJECT | TRAINER | |
| 08:30 – 10:30 | Disinfection and sterilization (methods and efficiency) | Kasper Moreaux |
| 10:30 – 10:50 | Coffee break | |
| 10:50 – 11:40 | Parallel session: Cleanroom essentials | |
| 25 min | Group 1: Microscopy | Magda Verfaillie |
| 25 min | Group 2: Consumables and use of USB microscope | Kasper Moreaux |
| 11:40 – 12:40 | Parallel session: Essential machines | |
| 30 min | Group 1: Autoclaves and steam generator, practical use of steam, cooling units | Pascale Vergeyle |
| 30 min | Group 2: Visit to the technical floor | Kasper Moreaux |
| 12:40 – 13:40 | Lunch break | |
| 13:40 – 14:40 | Dealing with micro-organisms in the production of spawn (presence of propagules in the air, cleanroom classes, air control, use of LAF, use of overpressure, rules of conduct) | Magda Verfaillie |
| 14:40 – 15:00 | Coffee break | |
| 15:00 – 16:20 | Mother cultures and mother spawn (GMP, quality control and degeneration of strains) | Kasper Moreaux |
| 16:20 – 17:00 | Case study: Examples from real life | Pascale Vergeyle |
| SUBJECT | TRAINER | |
| 08:30 – 13:00 | Parallel session: Inoculation practice | |
| 120 min | Group 1: Mother spawn department (pratical subculture method inoculation, strain preservation techniques, demonstration of incubating mother cultures) | Magda Verfaillie & Hélène Ntimba |
| 10:30 – 11:00 | Coffee break | |
| 120 min | Group 2: Spawn inoculation in Mycelia’s main inoculation room (essentials of large-scale production inoculation, particle counting and air speed measurement, incubation, shaking and reshaking, cleaning and hygiene) | Pascale Vergeyle |
| 13:00 – 14:00 | Lunch break | |
| 14:00 – 14:30 | Spawn conservation (spawn production and spawn quality, spawn testing, transport and conservation) | Magda Verfaillie |
| 14:30 – 15:30 | Demonstration of incubating spawn (good and bad examples, discussion) | Kasper Moreaux |
| 15:30 – 16:00 | Coffee break | |
| 16:00 – 17:00 | Spawn bags (types of vessels, bags and boxes, essentials of filters, spawn bags vs. production system) | Jan Van Nuffel |
| SUBJECT | TRAINER | |
| 08:30 – 10:00 | Feasibility study (financial study and investment) | Roel Van de Heyning |
| 10:00 – 11:20 | Practical: Organization of a spawn lab | Magda Verfaillie |
| 11:20 – 11:40 | Coffee break | |
| 11:40 – 12:40 | Practical: Cleanroom class | Jan Van Nuffel & Kasper Moreaux |
| 12:40 – 13:40 | Lunch break | |
| 13:40 – 16:00 | Parallel session: Cleanrooms practical | Pascale Vergeyle |
| 60 min | Group 1: Construction and maintenance of laminar air flows and overpressure units | |
| 60 min | Group 2: Practical cleaning (materials, disinfection, water management and practical hygiene) | |
| 14:40 – 15:00 | Coffee break | |
| 16:00 – 17:00 | Evaluation of petridishes and multiple choice of day 1, delivery of USB stick and certificates |
COMBO OFFER: 20% OFF
Combine this course with the Substrate and Mushroom Production course to get 20% discount.
1480 EUR + 1180 EUR = 2660 EUR 2128 EUR
Note: we organise a combo offer only once per year.
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